Part:BBa_K2387022:Design

Cpx system reporter + tethered CpxP

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 1454
  Illegal BglII site found at 2372
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 636
  Illegal AgeI site found at 748
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 1152
  

Design Notes

Strong tac promoter + strong RBS were chosen to increase inner membrane tethered CpxP production levels

Source

Cpx reporter mRFP1 under control of pCpxR originates from biobrick BBa_K339007

Tethered CpxP consists of misfolder MalE24-1 fused to N-terminus of CpxP, originating from biobrick BBa_K2387019.